Research finds bacterial protein captures viruses in contaminated water

Sponsored by

Dec. 16, 2011 -- Access to clean water is a necessity often taken for granted. However UNICEF estimates that 900 million people across the world do not have access to safe drinking water. New research published in BioMed Central's open access journal BMC Biotechnology shows that an enteric virus-binding protein (EVBP), isolated from bacteria found in activated sludge, is able to capture viruses often present in contaminated water.

One of the difficulties in measuring viral contamination in water is that viruses may be present at a very low concentration yet still make people ill. Even a single enteric virus can infect a human and cause gastroenteritis, and these viruses can survive for a long time in water.

Researchers from Tohoku University and Hokkaido University used activated sludge, produced during sewage treatment by aerating the sewage and allowing bacteria to breakdown organic material, as starting material in their search for a protein able to bind to enteric viruses. Using polymerase chain reaction (PCR) the researchers isolated the gene coding for one of the subunits of GroEL from sludge DNA. GroEL is a 14 subunit 'chaperone' protein which ensures that proteins are folded correctly during their manufacture.

Using biochemical and enzymatic assays the subunit was found to be able to capture enteric viruses. GroEL is able to bind to hydrophobic amino acids on the surface of proteins and it is thought that the newly isolated EVBP similarly binds to hydrophobic areas on the surfaces of viruses and viral fragments.

Dr Daisuke Sano from Hokkaido University explained, "Unlike virus-specific and expensive antibodies, EVBP bound all the enteric viruses we tested (norovirus, rotavirus and poliovirus). Once developed this easy-to-use method could be used to detect low concentrations of viruses in the clinic or environment."

For more information, read the full research article "Adsorption characteristics of an enteric virus-binding protein to norovirus, rotavirus and poliovirus" by Takahiro Imai, Daisuke Sano, Takayuki Miura, Satoshi Okabe, Keishi Wada, Yoshifumi Masago and Tatsuo Omura. BMC Biotechnology, December 2011.

###

Sponsored by

TODAY'S HEADLINES

CH2M HILL lauded for noteworthy wastewater treatment projects

CH2M HILL has been recognized with two Global Water Awards for its exceptional infrastructure work involving Peru's Taboada Wastewater Treatment Plant and the Bahrain Petroleum Company.

Winners of 2013 Campus RainWorks Challenge targeting green infrastructure announced

Four winners of the Environmental Protection Agency's second annual Campus RainWorks Challenge were recently announced.

S.F. Bay water quality, wetlands to be improved with $5M EPA grants

Nearly $5 million in grants provided by EPA have been designated to restore water quality and wetlands throughout the San Francisco Bay watershed.

Aeration Problem?

A supposed aeration problem is often nothing of the sort; it is simply the need for an efficient and appropriate mixer. Therefore, any facility striving to achieve as much treatment as possible on-site should consider mixing to reduce total operation costs.

FOLLOW US ON SOCIAL MEDIA